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    We carry out research for the e-liquids of tomorrow

    happy liquid research

    Since the founders of Happy People GmbH have a scientific background, it was a priority for us to incorporate science into the world of vaping from the very beginning. We try to collect research data relevant to e-cigarettes, whether in the basic research or in various special areas. happy liquid is the first e-liquid brand in the world to campaign for research in the area of e-liquids and e-cigarettes and publish their results nationally and internationally. All insights we gain on the topic of e-liquids and e-cigarettes are published here.

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    Studies

    Acute toxic effects of tobacco smoke compared to the e-liquid vapor on cultured human lung cells

    Long-term exposure of happy liquid on cultured human lung cells

    Examination of happy liquids for mutagenic effects in the Ames test

    Publications

    Innovations in Food Technology Feb. 2015: Tobacco smoke vs e-liquid vapour – Diverging toxic effects on human lung cells in culture

    Download

    Innovations in Food Technology Aug. 2015: Further in vitro studies on the cytotoxic effects of cigarette smoke vs. e-liquid vapour

    Download

    GFN 2015: Effect of cigarette smoke and e-liquid vapour on ciliary beat frequency (CBF) of freshly isolated human nasal epithelial cells

    Download

    GFN 2015: Acute and long-term cytotoxicity of cigarette smoke and e-liquid vapour on
    cultured human lung cells

    Download

    GFN 2015: Effects of cigarette smoke and e-liquid vapour on mass cultures of human lung cells:
    Continuous monitoring by time-lapse videography

    Download

    GFN 2016: Acute human lung cell toxicity of some selected flavouring chemicals
    after simulation of vaping

    Download

    GFN 2016: Acute toxicity of a flavoured e-liquid according to TPD 2 is related to e-cigarette vaporiser resistance and electrical power

    Download

    Yet research strives and struggles, never tiring,
    For the law, the reason, why and how.

    Johann Wolfgang von Goethe (1749 - 1832)
    german poet, scientist and statesman

    Videos

    Our research on human lung cell cultures has resulted in some videos. These were kindly made available to us by Prof. Dr. Peter C. Dartsch of Dartsch Scientific GmbH. The vapor of the tested e-liquids (also the smoke of the tobacco cigarette) was applied in aqueous solution to the cell cultures and the changes were photographed over a period of 10 hours. A time-lapse film was created from the footage in each case.

    (Time lapse footage with a Basler acA 1600-20uc digital camera combined with a Olympus-Inversmikroskop IX-50)

    Happy Liquid

    The vapor of the happy liquid "Strawberry-Menthol" was introduced into an aqueous medium. This medium was added to a culture of human lung cells and the cell behavior was recorded in time-lapse over a period of 10 hours. Here one sees no damage to the cell culture and recognizes normal cell divisions (mitoses), cell migration and only very few dying cells.

    e Liquid aus den USA

    For comparison, the vapor of an e liquid from the USA was introduced into an aqueous medium. This medium was added to a culture of human lung cells and the cell behavior was recorded in fast motion over a period of 10 hours. Here one can see clear damage and changes in the cells with cell divisions no longer proceeding in a regular manner as well as a clearly reduced cell migration. The appearance of many apoptotic and dying cells is particularly striking.

    Tabak

    Here, tobacco smoke was introduced into an aqueous medium. This medium was added to a culture of human lung cells and the cell behavior was recorded in time-lapse over a period of 10 hours. It can be clearly seen that after as little as 4 hours, the cells reacted by stopping cell division and cell migration, and then rounded off more. Finally, all cells have died.

    Cooperations

    In the field of science, Happy People GmbH cooperates with various research institutions worldwide. This serves the research of the e-cigarette, e-Liquids and the effects on humans and their health. We try to generate research data that will help all e cigarette users and thus advance this young field of research.

    Currently, we are cooperating with Prof. Dr. Riccardo Polosa (University of Catania) and LIAF (Lega Italiana Anti Fumo) and support their research financially.

    This resulted, among other things, in the open-source publication of the article:

    E-cigarettes and smoking cessation: a critique of a New England Journal Medicine-commissioned case study (Polosa, R. & Caponnetto, P. Intern Emerg Med (2016)).


     

    Interesting publications

    Interesting publications, research results and articles related to e cigarettes, e liquid, research and effect:

    • The well-known study on the positive impact of e cigarette from the health system of UK (2015). E-cigarettes: an evidence update
    • E-cigarettes and heated tobacco products: evidence review (2018).

    Do you have a question?

    Would you like to know more about our research projects or are you interested in the background? Maybe we can answer some of your possible questions here.

    Why is happy liquid the only German company actively researching e liquids?

    happy liquid has a very high standard since its foundation and sees itself in the pioneering role to establish meaningful standards and the highest possible product safety. Therefore, we not only research the finished product, but also individual substances as basic research!

    What does the research mean for the customer?

    Our customers can be sure to use the best product at any time. Now and in the future!

    Do animals come to harm during happy liquid research?

    No, we do not use animal testing to study the effects of e Liquid or vapor on the body. happy liquid is vaporized by humans, so we also use human lung cells to study effects and possible side effects.

    Have Questions?

    • info@happy-liquid.com
    • +49 (0)89 / 41 61 53 54

    Service telephone:
    Mon-Fri (workdays)

    from 09:30 am – 11:30 am
    and 1:00 pm – 5:00 pm

    We would be happy to help.

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    • Happy People GmbH
    • Rupert-Mayer-Str. 44,
    • 81379 München
    • Germany
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    • tel.: +49 89 41615354
    • fax.: +49.(0)89.41615356

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